FIG. 7.

Electrophoretic analysis of RNA extracted from Δ31 particles synthesized from either replicating or nonreplicating Δ31 RNA2 in the presence of RNA1 replication. Sf21 cells were infected with AcR1δ and either AcΔ31δ or AcΔ31[−3′UTR] at an MOI of 10. Five days postinfection, Δ31 particles were purified by sucrose gradient sedimentation and fractions containing T=3 particles were collected. RNA was extracted from the particles, and 1-μg aliquots were run on a nondenaturing agarose gel. RNA isolated from authentic FHV particles (vRNA) and Δ31 RNA2 transcripts (Δ31ts) were run in lanes 2 and 5 and lanes 3 and 6, respectively. The molecular sizes of RNA markers (in nucleotides) are indicated.