FIG. 8.

(A) Electrophoretic analysis of total cellular RNA confirming replication of AUG→Stop RNA2. Sf21 cells were infected with recombinant baculoviruses at an MOI of 10 as indicated. Three days after infection, total RNA was extracted from the cells and aliquots of 3 μg were run on a denaturing agarose gel, followed by staining with ethidium bromide. (B) Electrophoretic and Northern blot analyses of RNA packaged by coat protein derived from replicating versus nonreplicating RNA2. RNA was extracted from FHV particles synthesized in Sf21 cells infected with AcR1δ, AcR2δ[AUG→Stop], and either AcR2δ or AcR2[−5′3′UTR]. For electrophoretic analysis, 1-μg aliquots of this RNA were run on a nondenaturing agarose gel, while for Northern blot analysis, 5-ng aliquots were run on a denaturing agarose gel. Northern blot analysis was performed with a negative-sense RNA2 probe. RNA isolated from authentic FHV particles (vRNA) was included for comparison. The molecular sizes of RNA markers (in nucleotides) are indicated to the left.