Table 2.

List of differentially expressed miRNAs from miRNA PCR arrays using aqueous humor (AH) and lens capsule (LC) samples of patients with glaucoma with statistical significance, corresponding C_T values, and fold change data.

miRNA	AVG ΔCT		2-ΔCt		Fold change	p value
	Glaucoma	Control	Glaucoma	Control
AH
hsa-let-7a-5p	−9.64	−7.65	799.71	200.85	3.98	0.021
hsa-miR-125a-5p	−10.66	−7.99	1618.00	254.23	6.36	0.013
hsa-miR-1285-3p	−3.33	0.5	10.03	0.71	14.16	0.024
hsa-miR-181d-5p	−5.66	−2.16	50.56	4.46	11.34	0.017
hsa-miR-185-5p	−4.5	−1.66	22.58	3.16	7.14	0.012
hsa-miR-192-5p	−4.33	0.67	20.11	0.63	31.93	0.018
hsa-miR-193a-5p	−5.99	−3.33	63.70	10.08	6.32	0.005
hsa-miR-194-5p	−2.33	3.87	5.03	0.07	73.69	0.025
hsa-miR-195-5p	−6.66	−3.99	101.13	15.93	6.35	0.017
hsa-miR-210-3p	−5.67	−1.99	50.91	3.98	12.79	0.017
hsa-miR-221-3p	−5.32	−2.33	39.85	5.02	7.94	0.025
hsa-miR-222-3p	−5.82	−3	56.36	7.98	7.06	0.016
hsa-miR-25-3p	−6.99	−3.66	127.41	12.64	10.08	0.000
hsa-miR-31-5p	−4.82	−3	28.31	7.98	3.55	0.002
hsa-miR-365-3p	−5.67	−3.33	50.80	10.08	5.04	0.018
hsa-miR-512-5p	−3.32	4.18	10.01	0.06	181.44	0.017
hsa-miR-542-3p	1.01	4.82	0.50	0.04	14.06	0.006
hsa-miR-9-5p	−0.65	1.68	1.57	0.31	5.02	0.046
hsa-miR-92a-5p	−9.99	−6.33	1019.28	80.26	12.7	0.000
LC
hsa-let-7e-5p	−2.83	−0.74	7.093	1.6753	4.23	0.001226
hsa-miR-193a-5p	0.82	2.36	0.5661	0.1942	2.91	0.043613
hsa-miR-222-3p	2.71	4.51	0.1525	0.0438	3.48	0.027515

The miRNAs that showed significantly differential expression in the miRNA PCR array in both AH and LC samples of patients with glaucoma were miR-193a-5p and miR-222-3p. miRNA, microRNA; AH, aqueous humor; LC, lens capsule; ΔC_T, delta C_T; AVG ΔC_T, average ΔC_T; ΔC_T = C_T ([target gene] – C_T [reference gene]).