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. 2005 May;79(10):6052–6067. doi: 10.1128/JVI.79.10.6052-6067.2005

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FIG. 9. — Quantification of CD25⁺ cells in tissues from infected rabbits. Lymph node tissue sections from vMyx141KO (A and C)- and vMyxgfp (B and D)-infected rabbits were analyzed for both CD25 (red) expression and virus (green) infection and colocalization. Tissues were harvested and snap-frozen on days 3 (A and B) and 7 (C and D) postinfection and then were stained with an anti-rabbit CD25 monoclonal antibody as outlined in Materials and Methods. (E) Uninfected lymph node section. (F) Numbers of CD25⁺ cells present in lymph nodes of vMyx141KO- and vMyxgfp-infected rabbits at 3 and 7 days postinfection. The data shown are average numbers of positively staining cells for five randomly selected 40× fields of view (FOV) per tissue per rabbit, with two rabbits per group (for each virus and day postinfection).

FIG. 9. — Quantification of CD25⁺ cells in tissues from infected rabbits. Lymph node tissue sections from vMyx141KO (A and C)- and vMyxgfp (B and D)-infected rabbits were analyzed for both CD25 (red) expression and virus (green) infection and colocalization. Tissues were harvested and snap-frozen on days 3 (A and B) and 7 (C and D) postinfection and then were stained with an anti-rabbit CD25 monoclonal antibody as outlined in Materials and Methods. (E) Uninfected lymph node section. (F) Numbers of CD25⁺ cells present in lymph nodes of vMyx141KO- and vMyxgfp-infected rabbits at 3 and 7 days postinfection. The data shown are average numbers of positively staining cells for five randomly selected 40× fields of view (FOV) per tissue per rabbit, with two rabbits per group (for each virus and day postinfection).