TABLE 1.

Pathogenicity of vMyx141KO mutant myxoma virus in European rabbits

dpi	Exptl procedure or symptoms of infection
	Wild-type control virus (vMyxgfp)a	M141R deletion mutant virus (vMyx141KO)
0	Inoculation of four rabbits with 1,000 PFU vMyxgfp per site on each hind limb	Inoculation of three rabbits with 1,000 PFU vMyx141KO virus per site on each hind limb
3	Primary lesions at inoculation sites (1.4 to 2.0 cm) were pink and raised, conjunctivitis on one rabbit	Primary lesions at inoculation sites (0.9 to 1.3 cm), very slightly raised
7	Primary lesions were raised approximately 1 to 2 cm and were necrotic, with 6 to 14+ satellites (0.7 to 2.5 cm), thickened ears with 2 to 15+ coalescing lesions at base (0.5 to 4.0 cm), multiple lesions on nose (2.2 cm), 1 to 6 eye lesions (0.5 to 1.0 cm), copious exudate from eyes, 1 to 2 genital lesions, anxious behavior	Primary sites begin to cavitate, zero to four satellites appear (0.3 to 0.7 cm), one to three ear lesions (0.7 to 2.7 cm), inflamed eyelids, genital lesion on one rabbit (0.5 cm)
9	Primary lesions were extremely protuberant and severely necrotic (open wound), copious exudate from primary lesions, eight or completely coalescing thick purple ear lesions, multiple lesions on nose/lips, eyes swollen shut, one or multiple genital lesions (2.1 cm), very edematous head, rough fur, abnormal posture/activity, decreased food and water intake, decreased fecal output, dehydration, abnormal respiration, all rabbits scored as moribund and were euthanized	Well-formed primary lesion scabs zero to three satellites (0.6 to 1.0 cm), zero to two ear lesions (0.9 to 1.0 cm), one eye lesion each on two rabbits (0.4 cm), genital lesion on one rabbit (1.0 cm)
21	Not applicable	Primary lesions consisting of small, dry scabs, few scars remaining from secondary lesions, all rabbits immune to challenge with 2,000 PFU strain Lausanne per rabbit

^aThe symptoms listed were indistinguishable from those observed with vMyx141rev-infected rabbits.