Fig. 1. Isolation of cardiac endothelial cells.

(A) Endothelial cells were isolated from heart tissue for subsequent transcriptomic and epigenomic analyses via FACS using fluorescein-labeled lectin or via magnetic-assisted cell sorting (MACS) using anti-CD31 magnetic microbeads. (B) Cardiac endothelial cells were separated from other cardiac cell types by DRAQ5 positivity, forward light scatter, and fluorescein intensity. (C) Heatmap showing normalized gene expression of cell type–specific marker genes in heart tissue (13) and isolated cardiac endothelial cells as assessed by RNA sequencing (RNA-seq). FSC, forward scatter; FPKM, fragments per kilobase of transcript per million fragments mapped reads; ATAC-seq, assay for transposase-accessible chromatin with sequencing; ChIP-seq, chromatic sequencing; EC, endothelial cell; FACS, fluorescence-activated cell sorting.