Fig. 2. Gene expression and the epigenetic landscape in cardiac endothelial cells.

(A) Representative traces showing mRNA expression, accessible chromatin (ATAC) and histone marks in cardiac endothelial cells at the platelet endothelial cell adhesion molecule 1 (Pecam1) locus. (B) Active enhancer or (C) promoter regions were characterized by chromatin accessibility (ATAC), H3K27ac positivity, and H3K4me1 or H3K4me3 positivity, respectively. (D) Enrichment of transcription factor binding motifs in enhancer and promoter regions was analyzed using HOMER tools and correlated with the expression of the corresponding transcription factor gene. (E) Detailed representation. Further information on chromatin state analysis is provided in fig. S3. RPKM, reads per kilobase per million mapped reads; TF, transcription factor.