Fig. 4. Gene expression in cardiac endothelial cells in response to stress.

(A) Wild-type mice were subjected to TAC, ALDO infusion, Ang II infusion, ET1 infusion, IsoPE infusion, or streptozotocin (STZ) treatment, and cardiac endothelial cells were isolated by FACS. (B) Violin plots indicating differences in gene expression in endothelial cells from treated hearts compared to untreated CTRL (q < 0.05, n = 3 to 4 per group). Numbers of DEGs in one or more treatment conditions. (C) Genes that are differentially expressed in ≥4 conditions [defined here as common response genes] are highlighted. (D) Principal components analysis of all samples. (E) Enrichment of biological processes among common response genes as derived from gene ontology. (F) Enrichment of transcription factor binding motifs in enhancer regions connected to common response genes.