Figure 1.
Lz promotes pupal cell death. Anti-armadillo (Arm; green) marks the cell boundaries in 42-h-APF pupal retinas. The 2°, 3°, and bristle cells in B and F were traced and are shown to the right of the corresponding photograph (C and G, respectively). The cells in the trace are color-coded: bristle cells are dark green, 2° and 3° cells are blue, and extra 2° and 3° cells are orange; posterior is to the left. (L–P) Adult eyes of the indicated genotypes. (L,M) Male. (N–P) Female. For each genotype, the phenotype is consistent between individuals. (A, left) Timeline of pupal eye development. By 20 h APF the photoreceptors, cone, and 1° cells have differentiated. 2° and 3° cells differentiate shortly after PCD. (Right) One ommatidium from a 42-h-APF retina and corresponding trace with color-coded cells to show the relative location of each cell type. The ommatidia share three 3° cells at the apices of the ommatidial hexagon and six 2° cells between the bristle and 3° cells. (B,C) Wild-type (WT) pupal retina, in which a rare extra 2°/3° cell can be seen in the lower right corner. (D) Wild-type (WT) eye. (E) TUNEL (white) identified dying cells in a whole-mounted 32-h-APF wild-type (WT) retina. (F,G) Temperature-shifted lzts retina. (H) Temperature-shifted lzts adult eyes are only mildly rough and have wild-type pigmentation. (I) TUNEL (white) stain of a whole-mounted 32-h-APF temperature-shifted lzts pupal retina. (J,K) Separated channels of wild-type (WT) (J) and temperature-shifted lzts (K) retinas showing the expression of Hth (red) in the 2° and 3° (arrowheads) and bristle sensory organ, which is comprised of four cells (circle). Apically, Arm marks the cell boundaries, and basally, Hth marks nuclei. (J) There are no bristle cells in the upper left corner because this is a part of the rim of the eye. (L) GMR–hid. (M) Temperature-shifted lzts; GMR–hid. (N) GMR–rpr. (O) Temperature-shifted lzts/+; GMR–rpr. (P) Temperature-shifted lzts/+.