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. 1998 Oct;18(10):5880–5887. doi: 10.1128/mcb.18.10.5880

FIG. 2.

FIG. 2

Stimulation of C/EBPβ-mediated gene activation by TIF1β. (A) BHK cells were cotransfected with 2 μg of AGP (WT)-CAT, 0.5 μg of pCMV-C/EBPβ (C/EBPβ), and increasing amounts of pcDNA3-TIF1β (TIF1β) (1, 5, and 10 μg). (B) In 3.5-cm-diameter petri dishes, BHK cells were cotransfected with 0.5 μg of AGP (WT)-CAT, AGP (C mutant)-CAT, AGP (D mutant)-CAT, AGP (E mutant)-CAT, AGP (CDE mutant)-CAT, or AGP (GRE mutant)-CAT and 0.1 μg of pCMV-C/EBPβ with or without 2 μg of pcDNA3-TIF1β. The data represent the average activity of two independent duplicate experiments. The fold induction by TIF1β is indicated below the panel. mt, mutant. (C) (Left panel) In 3.5-cm-diameter petri dishes, BHK cells were cotransfected with 0.5 μg of AGP (WT)-CAT and 0.05 μg of pCMV-C/EBPα in the presence or absence of 2 μg of pcDNA3-TIF1β. (Right panel) BHK cells were transfected with 0.25 μg of pC/EBPβ-CAT, and 0.1 μg of pCMV-C/EBPβ or both 0.1 μg of pRSV-CREB and 0.1 μg of pCMV-PKAc in the absence or presence of 2 μg of pcDNA3-TIF1β. Error bars indicate standard deviations. −, pcDNA3 vector control.