FIG. 4.

TIF1β potentiates GR-activated gene expression. (A) HeLa cells were transiently transfected with 2 μg of pMMTV-CAT reporter plasmid, 1 μg of pRSV-hGR (GR), and increasing amounts of pcDNA3-TIF1β (TIF1β) (1, 5, and 10 μg). Each assay was done in the presence of 1 μM dexamethasone. (B) BHK cells were transiently transfected with 2 μg of AGP (WT)-CAT reporter plasmid. Other plasmids and conditions of treatment are as described for panel A. Relative CAT activity normalized with an internal control represents an average of two independent duplicate experiments. Error bars indicate standard deviations.