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. 2005 May 1;19(9):1067–1080. doi: 10.1101/gad.1291905

Figure 7.

Figure 7.

Isolated, endogenous GY-boxes, Brd-boxes, and K-boxes are sufficient and necessary for miRNA-mediated regulation. These discs contain ptc-Gal4>UAS-DsRed-miR-7 (A,B), dpp-Gal4>UAS-DsRed-miR-79 (C,D), dpp-Gal4>UAS-DsRed-miR-4, miR-5, miR-286 (E,F), dpp-Gal4>UAS-DsRed-miR-11 (G), along with tub>GFP sensors containing two copies of GY-box, Brd-box, and K-box sites derived from Bob, Bearded, and E(spl)m8, respectively. Note that the GY-box sensor contains both GY-boxes of Bob (Fig. 3J); miRNA pairing is shown only for the more complementary site. (H,J) Expression of a miR-6 sensor in dpp-Gal4>UAS-miR-6-1, dpp-Gal4>UAS-miR-6-2, dpp-Gal4>UAS-miR-6-3 (miR-6) (H), dpp-Gal4>UAS-miR-2a-1, dpp-Gal4>UAS-miR-2a-2, dpp-Gal4>UAS-miR-2b-2 (miR-2) (I), and dpp-Gal4>UAS-DsRed-miR-11 (mir-11) (J) backgrounds.