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. 2024 Feb 26;13:e94694. doi: 10.7554/eLife.94694

Figure 6. Levels of centrosomal components are reduced upon neural differentiation.

Figure 6.

(A) Immunostaining for TUBG and CEP152, TUBG and SAS-4, or TUBG and STIL in wild-type (WT) mouse embryonic stem cells (mESCs) and in vitro differentiated (neural progenitor cells, NPCs). Insets are magnifications of the center of the dashed squares. Scale bars = 3 µm and 1 µm (insets). (B) Quantification of the centrosomal TUBG and CEP152 signal from (A). Values were normalized to mESCs. Error bars represent mean ± s.d. Quantification of TUBG, mESCs: 1.00 ± 0.2 (n=1325 centrosomes from four independent experiments); NPCs: 0.03 ± 0.02% (n=789 from 4fourindependent experiments). Quantification of CEP152, mESCs: 1.00 ± 0.2 (n=1006 cells from five independent experiments); NPCs: 0.2 ± 0.04% (n=973 from five independent experiments). **p<0.01, ***p<0.001 (two-tailed Student’s t-test). (C) Quantification of the centrosomal SAS-4 signal from (A) from four independent experiments. Values were normalized to mESCs. Error bars represent mean ± SD mESCs: 1.00 ± 0.1 (n=1297 centrosomes); NPCs: 0.2 ± 0.08% (n=790). ****p<0.0001, (two-tailed Student’s t-test). (D) Quantification of the centrosomal STIL signal from (A) from four independent experiments. Values were normalized to mESCs. Error bars represent mean ± SD mESCs: 1.00 ± 0.13 (n=1132 centrosomes from four independent experiments); NPCs: 0.3 ± 0.07% (n=798). ***p<0.001, (two-tailed Student’s t-test).