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. 2024 Feb 26;13:e94694. doi: 10.7554/eLife.94694

Figure 7. SAS-6-independent centriole formation in mouse embryonic stem cells (mESCs) depends on a threshold Polo-Like Kinase 4 (PLK4) activity.

Figure 7.

(A) Immunostaining for TUBG and FOP in wild-type (WT) and Sass6−/− mESCs treated for 4 days with DMSO, 100 nM or 500 nM centrinone B. Insets are magnifications of the center of the dashed squares and show the representative image of the majority of population. Scale bars = 5 µm and 1 µm (insets). (B) Quantification of the percentage of cells with centrosomes (TUBG and FOP) from (A) from four independent experiments. Error bars represent mean ± SD WT, DMSO: 90 ± 12% (n=5280 cells), 100 nM: 82 ± 15% (n=6083 cells), 500 nM: 10 ± 4% (n=4809 cells); Sass6−/−, DMSO: 46 ± 12% (n=5786 cells), 100 nM: 6 ± 4% (n=7502 cells), 500 nM: 4 ± 2% (n=6220 cells). ***p<0.001, ****p<0.0001, ns = not significant with p>0.05 (one-way ANOVA with Tukey’s multiple comparisons).