Table 1. Description of CRISPR/Cas9-mediated knockouts of Sass6 in the mouse in vivo.
| Sass6em4/em4 | Sass6em5/em5 | |
|---|---|---|
| Location | exon 4 | exon 5 |
| gRNA | 5′-GGTGGACTTCTTAGCTTTCC-3′ | 5′-ACCGGTCCTTTTAAACGTAG-3′ |
| Change | 3 bp del and 1 bp insertion (a net of 2 bp deletion) | 5 bp del |
| Mutation |
NC_000069.7(Chr3)*: g.116399341_116399343delinsC |
NC_000069.7(Chr3)*: g.116401034_116399338del |
| InDel | GGTCTTCTTAGCTTTCC | ACCGGTCCTTTTAAACG |
| Predicted STOP codon | 130 amino acids downstream of the translation start site, with 78 amino acids not native to the protein | 129 amino acids downstream of the translation start site, with six amino acids not native to the protein |
*
RefSeq sequence number from GRCm39 assembly, NCBI annotation release 109.