Table 2. Description of CRISPR/Cas9 mediated knockout of Sass6 in mouse embryonic stem cells (mESCs).

	Sass6−/− mESCs
Location	Deletion of entire ORF of Sass6
gRNAs	5′-TAACAAACGTGGCCGCCTGA-3′ 5′-ACCAAGCCTGAGTTACACAA-3′
Change	34,524 bp deletion
Mutation	NC_000069.7(Chr3)*:g.116388519_116423042del
Predicted protein	No predicted protein expression

^*RefSeq sequence number from GRCm39 assembly, NCBI annotation release 109.