Figure 5.

RNAi to FEZ1 reduces the resistance of the mutant R3-2 line. Wild-type or mutant R3-2 cells were transfected with three independent short-interfering RNAs (indicated as A, B, or C) on two consecutive days with equal amounts of RNA duplexes and subsequently seeded and infected with various amounts of MLV-N-neo. Cells were selected in medium containing G418, and the number of the transduced colonies was then counted after 8–12 d. The wild-type Rat2 cells and a non-specific GFP duplex were included as negative controls. Similar results were obtained in at least three independent experiments.