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. 2005 May 1;19(9):1116–1127. doi: 10.1101/gad.1297105

Figure 1.

Figure 1.

GR differentially regulates NFκB at the IL-8 and IκBα genes in A549 cells. (A) Steady-state IL-8 and IκBα mRNA levels are stimulated in A549 cells treated for 2 h with TNFα (2.5 ng/mL). Cotreatment with TNFα and dex (100 nM) selectively repressed IL-8 mRNA accumulation. (B) A549 cells were transfected with IL-8-Luc, IκB-Luc, IL-8mut-Luc, or IκBmut-Luc (50 ng) reporter genes and RSV-βgal (50 ng) followed by treatment with combinations of TNFα and dex as indicated for 5 h. Luciferase units were normalized to β-galactosidase activity. (C) Diagram of the human IL-8 and IκBα genes. The promoter proximal κB site sequences are boxed.