Table 2.

HPLC conditions for flavonoids.

Instrument	Shimadzu, Japan
Mobile phase	(A) solution: 1% acetic acid (B) Solution : acetonitrile : water 80% in ratio of 90% B : A
Particle size	5 μm
Column	ODS C18 (250 mm × 4.6 mm internal diameter)
Flow rate	1.2 mL/min
Column temperature	Room temperature
Injection volume	20 μL
Injection concentration	1 μg/mL
Detection wavelength	UV-Vis at wavelength of 254 nm
Standards used	Rutin, caffeic acid, apigenin, hesperetin, kaempferol, coumarin, and myricetin