FIG. 5.

NM1 and IGF-IR activities are not inhibited by PKC-δK376R expression. (A) NIH 3T3 transfectants were serum starved overnight in DMEM and either untreated or stimulated with 10 ng of IGF-I per ml for 10 min. Equivalent cell lysates were immunoprecipitated with anti-IGF-IR serum, and transferred proteins were immunoblotted with anti-pTyr. (B) NIH 3T3 transfectants were treated in a manner similar to that described for panel A. Equivalent cell lysates were immunoprecipitated with anti-IGF-IR serum and subjected to an immune complex assay as described in Materials and Methods. The dried gel was autoradiographed. An 80-kDa phosphoprotein associated with NM1 is indicated by the asterisk. (C) NIH 3T3 transfectants were treated in a manner similar to that described for panel A. Equivalent cell lysates (100 μg per lane) were subjected to immunoblot analysis with anti-pTyr. Marker proteins are given in kilodaltons. IP, immunoprecipitation.