Figure 4.

Auxin Stimulates Adventitious Roots on Etiolated ago1 Seedlings but Never as in the Wild Type.

Emergent adventitious roots were scored 7 d after transfer to the light. At least 40 seedlings were used for each data point. This was repeated on three independent biological replicates.

(A) Siblings from a heterozygous ago1-3 mother plant were germinated and grown in the dark for different times, then transferred to the light. The proportion of seedlings forming one or more adventitious roots was determined after 1 week in the light.

(B) Proportion of wild-type and mutant seedlings forming one or more adventitious roots after transfer to the light for 1 week on a medium with or without 1-NAA.

(C) Average number of adventitious roots formed on wild-type or mutant seedlings in the absence or presence of 1-NAA. Error bars indicate se.

(D) Average number of adventitious roots formed on Col-0, sur2, the five ago1 mutants, and the five sur2 ago1 double mutants after etiolation and transfer to the light for 1 week. Error bars indicate se.