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. 2005 May;17(5):1343–1359. doi: 10.1105/tpc.105.031625

Figure 6.

Figure 6.

Free IAA Content in Col-0, ago1-3, sur2-1(hyb), and ago1-3 sur2-1(hyb) Seedlings.

The endogenous free IAA level was measured in seedlings germinated and grown for 2.5 d in the dark, then transferred to the light for 24, 48, or 72 h. FW, fresh weight.

(A) Entire seedlings. Free IAA content was lower in ago1-3 than in the wild type (P < 0.5). The sur2-1 ago1-3(hyb) double mutant contained significantly more auxin than the wild type (P < 0.01) but less than sur2-1(hyb) (P < 0.01).

(B) Root. The auxin content increased in all the genotypes after transfer to the light. No significant difference between ago1-3 and Col-0 or sur2-1(hyb) and sur2-1 ago1-3(hyb) could be detected.

(C) Apical part (cotyledons plus hypocotyl). The auxin content decreased significantly in ago1-3 seedlings after transfer to the light (P < 0.01) and was significantly lower than that in the wild type after 72 h in the light (P < 0.01). The auxin content increased significantly in sur2-1(hyb) after transfer to the light (P < 0.05) but decreased in sur2-1 ago1-3(hyb) (P < 0.05).

Three biological replicates were used for each data point. Error bars indicate sd. A t test was performed according to http://graphpad.com/quickcalcs/ttest1.cfm.