Figure 6.

IAA Treatment of Wild-Type and 5mARF17 Plants.

(A) Primary root length of 8-d-old Col-0 and T2 seedlings mock treated (white bars) or treated with 50 nM IAA (black bars). 5mARF17 progeny with wild-type phenotypes (−) lacked the transgene; those with mild (+) or severe (++) phenotypes scored positive for the 5mARF17 transgene. The number of plants analyzed is indicated above each bar, and standard deviations are shown (except in cases where fewer than three plants were analyzed).

(B) Hypocotyl length of 7-d-old Col-0 and T2 seedlings mock treated (white bars) or treated with 0.1 μM IAA (gray bars) and 10 μM IAA (black bars). 5mARF17 progeny with wild-type phenotypes (−) lacked the transgene; those with mild (+) or severe (++) phenotypes scored positive for the 5mARF17 transgene. The number of plants analyzed is indicated above each bar, and standard deviations are shown (except in cases where fewer than three plants were analyzed).

(C) RNA gel blot analysis of 10 μg total RNA prepared from IAA treated (+) or untreated (−) Col-0 seedlings with DNA probes complementary to miR160, miR164, miR167, or U6 (loading control). Blots were stripped between each hybridization. The positions of ³²P-labeled RNA oligonucleotides are noted at the right. Also shown are RNA gel blot analyses of 10 μg of the same RNA with RNA probes complementary to IAA1, IAA19, or 3′ ARF17. The positions of each full-length transcript and ARF17 3′ cleavage product are noted at the left. The 25S rRNA is shown as a loading control. Normalized values of miRNA to U6 RNA and IAA1, IAA19, and ARF17 full-length mRNAs to ACTIN2 mRNA (with untreated Col-0 levels set at 1.0) and ratios of ARF17 full-length mRNA to 3′ cleavage product are indicated.