Figure 8.

GH3-Like Gene Expression Changes in 5mARF17 Rosette Leaves.

(A) RNA gel blot analysis of 7 μg of total RNA from 30-d-old rosette leaves with RNA probes complementary to GH3.3, YDK1/GH3.2, DFL2/GH3.10, IAA1, or 3′ ARF17. The absence (−) or presence of a severe (++) rosette leaf phenotype is indicated. The positions of each full-length transcript and ARF17 3′ cleavage product are noted at the right. The 25S rRNA is shown as a loading control. Normalized values of mRNAs to ACTIN2 mRNA are indicated, with mRNA levels in Col-0 control plants set at 1.0. Because YDK1 transcripts were not detected (ND) in Col-0 or ARF17 control rosette leaves, the approximately fourfold increase in YDK1 signal observed represents a minimum.

(B) Similar analysis with probes complementary to GH3.5 or DFL1/GH3.6. These transcripts were not detected in 5mARF17 rosette leaves, so the fold changes observed represent a minimum.

(C) RNA gel blot analysis of 7 μg of total RNA from 30-d-old rosette leaves with RNA probes complementary to GUS or 3′ ARF17. The absence (−) or presence of a mild (+) or severe (++) rosette leaf phenotype is indicated. The positions of each full-length transcript and ARF17 3′ cleavage product are noted at the right. Normalization is as in (A).

(D) Fifteen-day-old ARF17 DR5-GUS and 5mARF17 DR5-GUS cotyledons.

(E) Twenty-eight-day-old ARF17 DR5-GUS and 5mARF17 DR5-GUS rosette leaves with moderate (top) or more severe (bottom) leaf defects.