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. 2005 May;17(5):1376–1386. doi: 10.1105/tpc.105.030841

Figure 1.

Figure 1.

Expression Patterns of NAC1 and miR164 in Wild-Type Arabidopsis RNA.

Gel blot analyses of NAC1 mRNA and miR164 from wild-type (Col) leaves (L), inflorescences (I), 2-week-old seedling rosette leaves (Ro), and roots (Rt). A gel blot containing total RNA (10 μg per lane) was hybridized with a NAC1 3′-specific fragment (nucleotides 682 to 1063; top gel). Intact NAC1 mRNA and the NAC1 3′ fragment resulting from miR164-guided cleavage are indicated. 28S rRNA visualized by methylene blue staining was used as a loading control (second gel). A gel blot containing low-molecular-weight RNA (30 μg per lane) was hybridized with an end-labeled DNA oligonucleotide complementary to miR164 (third gel). The membrane was stripped and reprobed with an end-labeled DNA oligonucleotide complementary to miR163 for a loading control (bottom gel).