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. 2023 Nov 15;3(1):4. doi: 10.20517/mrr.2023.02

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© The Author(s) 2023.

© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Gram-negative and Gram-positive bacterial ratio in different biological samples before and after saponin-based depletion protocol. Panel (A) indicates the percentages of Gram-negative (pink) and Gram-positive bacteria (green) on the total amount of bacterial DNA profiled in each biological sample before (NT) and after 2.5% wt/vol saponin-based depletion (T); panel (B) represents the percentages of Gram-negative (pink) and Gram-positive bacteria (green) detected in a sputum sample treated with different saponin amounts.