Table 2.
Experimental Verification of Previously Predicted miRNAs in Rice
| Gene | miRNA Sequence (5′→3′) | Cloning Frequencies in Libraries |
|---|---|---|
| OsmiR156a-j | UGACAGAAGAGAGUGAGCAC | Shoot (12), root (5) |
| OsmiR156k,l | UGACAGAAGAGAGUGAGCACA | Shoot (3), root (1), inflorescence (1) |
| OsmiR 159a,b | UUUGGAUUGAAGGGAGCUCU | Shoot (3), inflorescence (2) |
| OsmiR159c | UUGGAUUGAAGGGAGCUCUGC | Shoot (2) |
| OsmiR160 | UGCCUGGCUCCCUGUAUGCCA | Shoot (1) |
| OsmiR164 | AUGGAGAAGCAGGGCACGUGCA | Shoot (3), inflorescence (3) |
| OsmiR166a-f | UCGGACCAGGCUUCAUUCCCC | Shoot (2), inflorescence (1) |
| OsmiR167a,b,c | UGAAGCUGCCAGCAUGAUCUA | Shoot (2) |
| OsmiR167d-I | UGAAGCUGCCAGCAUGAUCUG | Root (1) |
| OsmiR168a | UCGCUUGGUGCAGAUCGGGAC | Shoot (14), root (3), inflorescence (5) |
| OsmiR169b,c | CAGCCAAGGAUGACUUGCCGG | Shoot (2) |
| OsmiR169f,g | UAGCCAAGGAUGACUUGCCUA | Shoot (2), root (1) |
| OsmiR169 h-m | UAGCCAAGGAUGACUUGCCUG | Shoot (1) |
| OsmiR171a-f | UGAUUGAGCCGUGCCAAUAUC | Shoot (1), root (1), inflorescence (2) |
| OsmiR171g | GAGGUGAGCCGUGCCAAUAUC | Root (1) |
| OsmiR172a | AGAAUCUUGAUGAUGCUGCAU | Shoot (2), inflorescence (2) |
| OsmiR393 | UCCAAAGGGAUCGCAUUGAUC | Inflorescence (1) |
| OsmiR397b | UUAUUGAGUGCAGCGUUGAUG | Shoot (1), root (1) |
| OsmiR398 | UGUGUUCUCAGGUCGCCCCUG | Shoot (1) |
| OsmiR399a | UGCCAAAGGAGAAUUGCCCUG | Shoot (1) |
| OsmiR408 | CUGCACUGCCUCUUCCCUGGC | Root (1) |