Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 1;36:83–95. doi: 10.1016/j.bioactmat.2024.02.030

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 2 — Characterization of cellular uptake, endosome escape, and transfection efficiency of TPH NCs. (A) Cellular uptake of RA FLS treated YOYO-1 labeled PEI25 K/pDNA, ^TKPF/pDNA and TPH NCs by Flow cytometry analysis. (B) Quantitative cellular uptake efficiency and (C) mean fluorescence intensity of PEI25 K/pDNA, ^TKPF/pDNA, and TPH detected by flow cytometry. (D) Fluorescence microscopy images of RA FLS after incubation with PEI25 K/pDNA, ^TKPF/pDNA, and TPH. Scale bar: 200 μm. (E) CLSM images of RA FLS incubated with TPH NCs. Scale bar: 50 μm. (F) Fluorescence images of RA FLS treated by PEI25 K/pDNA, ^TKPF/pDNA and TPH NCs using pEGFP after transfection for 24 h. Scale bar: 200 μm. (G) Quantitative transfection efficiency result analyzed through flow cytometry. (H) Mean fluorescence intensity by flow cytometry. Data are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.