Extended Data Fig. 6. Dscc1 mouse embryonic fibroblasts (MEFs) grow slower and show increased genomic instability.
a, Bar graph quantifying the incorporation of 5-Ethynyl-2’-deoxyuridine (EdU) in MEFs of the indicated genotypes shows no difference in the S-phase cell cycle fraction. Significance was assessed using a Student’s two-tailed t-test. Experiment performed three independent times (n = 3 biological replicates). Mean is shown with the error bars denoting s.d. b, Cell growth profiles (left) for cell lines derived from the same E13.5 litter show that Dscc1−/− MEFs grow significantly slower than wild-type (WT) controls. For a,b, two independent WT and two independent Dscc1−/− MEF lines were derived from littermate embryos; n = 3 independent wells/replicates each. Bars represent mean with s.e.m. Significance was assessed using an Student’s two-tailed t-test comparing the area under the curve (AUC). c, Fluorescent in-situ hybridization (FISH) images of metaphases from MEFs derived from littermates showing increased chromosomal aberrations characteristic in Dscc1−/− cells. This experiment was replicated three independent times. Size bar 10 µm. This image is a lower magnification of the image shown in Fig. 2d.