Extended Data Fig. 10. Deficiency in adjusting axonal conduction speed following energy deprivation.

a, Time course of CAP area changes from optic nerves of ∼3 months old cKO (n = 6) and ctrl mice (n = 6), challenged with glucose deprivation (GD) and mitochondrial inhibition (MI) using 5 mM NaN₃ (GD + MI, simulating chemical ischemia). See also Fig. 5b. Inset (bottom right) illustrates the CAP area. b, Partial CAP (pCAP) area (inset, bottom right) time course analysis during and after GD + MI. Note differing recovery kinetics between genotypes. c, Example traces of CAP response recovery (depicted in 25-second intervals, from blue to red), illustrating the first 10 minutes post GD + MI for ctrl (left) and cKO (right). Note the more considerable shift in peak 2 latency (indicated by arrow at dashed lines) during recovery in ctrl compared to cKO. d, Peak 2 latency analysis, adjusted to initial baseline value before GD + MI. Notably, post GD + MI, the initial increase in peak 2 latency of the first recovering CAPs was similar in both genotypes; however, its return to normal latency was significantly faster in ctrl than in cKO (n = 6, p < 0.0001, two-way ANOVA). Data represented as means ± SEM.