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. 2005 May;17(5):1482–1496. doi: 10.1105/tpc.105.030700

Figure 4.

Figure 4.

Construction and Expression of atTic110 Deletion Mutants in Arabidopsis.

(A) Schematic diagram of the atTic110 constructs used in this study. The known structural and functional regions of atTic110 are indicated. The numbers refer to the amino acid position, with 1 indicating the N-terminal residue of mature atTic110.

(B) Immunoblots of total protein extracts from transgenic Arabidopsis overexpressing the truncated proteins indicated at the top of the figures. The immunoblots were probed with anti-atTic110 (both panels) or anti-GFP (right panel) sera.

(C) Trypsin sensitivity of atTic110 and the atTic110 deletion constructs in isolated intact chloroplasts. Isolated chloroplasts were treated with the indicated concentrations of trypsin on ice for 30 min. Intact chloroplasts were reisolated, resolved by SDS-PAGE, and probed with anti-atTic110, anti-GFP, and anti-atToc75.