Figure 2.

Expression of ZmK2.1 in the Plant.

(A) RNA gel blot analysis. The blot (10 μg of total RNA per lane) was hybridized with ³²P-labeled ZmK2.1 probe.

(B) RT-PCR analysis. r, root; l, leaf without central vascular/bundle sheath strand; e, epidermis; v, central vascular/bundle sheath strand; gen, genomic DNA. Histone H1 was used as a control.

(C) Comparison of ZmK2.1 and KZM2 expression by RT-PCR analysis. l, leaf; r, e, and gen, same as in (B). Actin was used as a control. The actual amplification of KZM2 (GenBank accession number AJ558238) cDNA was checked by sequencing the PCR products.