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. 2005 May;17(5):1549–1558. doi: 10.1105/tpc.105.031567

Figure 1.

Figure 1.

AtMBD5, 6, and 7 Are Localized to Highly Methylated Chromocenters.

(A) Immunolabeling/FISH assay showing that 5-methylcytosine signals are associated with CEN180 at chromocenters in wild-type Arabidopsis.

(B) Transient expression showing different patterns of subnuclear localization of AtMBD-GFP proteins. Arabidopsis protoplasts were polyethylene glycol–transformed with the indicated AtMBD-GFP constructs and inspected under a confocal microscope. Note the preference of AtMBD5 and 6 for two perinucleolar chromocenters. Bars = 5 μm.

(C) Subnuclear localization of AtMBD6-GFP in various types of leaf cells in transgenic plants. Bars = 5 μm.

(D) Immunolabeling assay showing the localization of AtMBD6-GFP at the intensely DAPI-stained chromocenters. Fixed nuclei from Arabidopsis plants expressing AtMBD6-GFP were immunolabeled with anti-GFP and inspected under a fluorescence microscope. Note the intense GFP signal at perinucleolar chromocenters and the lower signals at other chromocenters.