Figure 3.

Localization of AtMBD-GFPs to Chromocenters Requires CpG Methylation.

(A) Subnuclear localization of AtMBD-GFPs is disrupted in ddm1-2 and met1-1 but not in the cmt3 mutant. The indicated AtMBD-GFPs were transiently expressed in ddm1-2, met1-1, and cmt3 mutant cells. Note the dispersion of AtMBD5 and 6 throughout the nucleus in ddm1-2 and to a lesser extent in met1-1. Also note that AtMBD2 accumulates at chromocenters in ddm1-2 and met1-1 but not in cmt3 cells. Bar = 5 μm.

(B) AtMBD2 is associated with CEN180 in the ddm1-2 mutant. Immunolabeling (anti-GFP) followed by FISH (CEN180) was performed on nuclei derived from transgenic ddm1-2 expressing AtMBD2-GFP.