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Reduced Methylation of Histone H3 at Lys 9 Does Not Affect AtMBDs Localization at Chromocenters.
(A) Immunolabeling using anti-Lys 9 dimethylated histone H3 (K9m2H3) showing intense labeling at chromocenters in the wild-type nucleus and reduced labeling in the kyp-2 nucleus. Arrows indicate nucleoli. Bars = 5 μm.
(B) Localization of AtMBD-GFPs in kyp-2 cells is indistinguishable from that of wild-type cells. nuc, nucleolus.
