Figure 1.
Domain Structures of the AtCAO and GFP Proteins and the CAO-GFP Fusion Proteins.
Nagata et al. (2004) reported the domain structure of AtCAO, assuming that the N-terminal 36 residues exist as a transit peptide. We used the N-terminal 56 residues to guide the fusion proteins into chloroplasts (see Results). A, A domain; B, B domain; C, C domain; GFP, sGFP (S65T); tp, N-terminal 56 residues of AtCAO.
(A) Domain structures of the AtCAO and GFP proteins.
(B) Structures of the CAO-GFP fusion proteins.