FIG. 1.

Rep functional domains. Within the Rep gene, two promoters, which are located at map units 5 and 19, direct the expression of four proteins, Rep78 and Rep68 and Rep52 and Rep40, respectively. Two proteins are produced from each promoter, one of which is spliced (Rep68 and Rep40) and one of which is unspliced (Rep78 and Rep52). Within the Rep ORF, several functional domains have been identified and are indicated at the top. These include the DNA DB, terminal resolution site (Y), NTP binding pocket (NTP), helicase domain (HD), nuclear localization domain (NLS), zinc finger (Zn), and splice sites, with their relative amino acid positions indicated below. The four Rep proteins as well as the portion of Rep used as bait in the yeast two-hybrid screen are indicated.