FIG. 3.

In vitro coimmunoprecipitation. Rabbit reticulocyte lysates programmed with Rep52 (lanes 1, 5, and 9) Rep68Δ (lanes 2, 6, and 10) luciferase (lanes 3, 7, and 11), or a random library clone (1c) (lanes 4, 8, and 12) were incubated in the presence of glutathione beads alone (lanes 5 to 8) or were supplemented with a GST-PrKX fusion protein (lanes 9 to 12). The bound protein was then precipitated and separated on a polyacrylamide gel. As a control, total protein in the programmed reticulocyte lysates was also analyzed (lanes 1 to 4). The products of the in vitro translations of Rep68 and Rep52 are indicated by the upper and lower arrows, respectively, on the left.