FIG. 8.

Interaction of MBP-Rep52 and MBP-Rep68 with immobilized GST-PKA and inhibition. (A) A total of 500 to 600 RU of GST-PrKX was immobilized via an anti-GST antibody to a CM5 chip. MBP-Rep52 (upper panel) and MBP-Rep68 (lower panel) at the concentrations indicated were injected for 240 s (association phase). After the injection was stopped, the dissociation rate was monitored for 300 s (dissociation phase). (B) GST-PKA (10 nM) was inhibited with Rep52 (checkered bars) and Rep68 (open bars) by using the concentrations indicated. Mean values with standard deviations of several different experiments are shown. The inhibitions were determined by the radioactive kinase assay in duplicate. Three time points for each Rep concentration were assayed.