FIG. 9.

Effect of Rep68 and Rep78 on cellular protein expression. Whole-cell lysate was prepared from cos cells transfected with either Rep68 or Rep78 expression vector or pUC as a control. Equal loading of protein was measured by Coomassie staining of duplicate gels. Western blots were probed with antibodies to Rep, CREB, or cyclin A (A, B, and C). As an additional control, the blots were also probed with actin (D). Horseradish peroxidase-conjugated secondary antibodies were used and detected by chemiluminescence. Changes in expression were determined by laser densitometry of the films and are reported relative to the level of expression observed in Rep68 or pUC.