Table 2.

ST, carbapenemase, antimicrobial susceptibility testing, and mutational ß-lactam resistance genotype of imipenem/relebactam-resistant and susceptible K. pneumoniae

Strain no.*	ST	CP	MIC (mg/L)								ß-lactam resistance genotype†
			MEM	AK	CIP	COL	TIG	IPM	IMR	CZA	ß-lactalactamase	OmpK351	OmpK36	OmpK372	AcrAB-TolC (RamR)	PBP13	PBP24
2015351	258	KPC-3	>64	>32	>2	0.25	1	>64	4	4	SHV-11	mut	wt	mut	wt	mut	mut
20171161	512	KPC-3	16	16	>2	2	1	8	4	0.5	SHV-11	mut	wt	mut	wt	mut	mut
20190807	512	KPC-3	>64	>32	>2	>16	>2	>64	4	8	SHV-11	wt	wt	mut	wt	mut	mut
20190822	512	KPC-3	>64	>32	>2	>16	2	>64	4	4	SHV-11, TEM-1	wt	wt	mut	wt	mut	mut
2016080	258	KPC-3	8	>32	>2	0.5	1	8	0.06	1	SHV-11	mut	wt	mut	wt	mut	mut
2017964	512	KPC-3	>64	>32	>2	1	2	64	0.125	8	SHV-11	mut	wt	mut	wt	mut	mut
20180393	512	KPC-3	>64	>32	>2	>16	1	64	0.25	2	SHV-11	mut	wt	mut	wt	mut	mut
20180260	512	KPC-3	>64	>32	>2	>16	2	>64	0.5	4	SHV-11	mut	wt	mut	wt	mut	mut
20180214	512	KPC-3	>64	>32	>2	>16	2	>64	1	8	SHV-11	mut	wt	mut	wt	mut	mut
2016252	512	KPC-23	>64	>32	>2	1	2	64	2	8	SHV-11, TEM-1	mut	wt	mut	wt	mut	mut

MEM meropenem, AK amikacin, CIP ciprofloxacin, COL colistin, TIG tigecycline, IMP imipenem, IMR imipenem/relebactam, CZA ceftazidime/avibactam, PBP penicillin binding protein, mut mutation detected, wt wild type

*One susceptible isolate has been included for each MIC value obtained

†K. pneumoniae ATCC10031 was used as reference to compared porin, PBPs, and efflux bomb aminoacid sequences

¹M2_Y36del, G37E, M39W, V40S

²N230G, M233Q, M233_T234insHYTH, Q235E, T236R, N238Y, R240K, E244D, N247S, D275T, D275_G276insSSTNGG, V277I

³I17V, V296_P297insPAQ, V297P, H657R, S662R

⁴D98G, T348S, G515S