Skip to main content
. 2024 Feb 20;27(3):109281. doi: 10.1016/j.isci.2024.109281

Figure 2.

Figure 2

The alteration of PCx proteome between WT vs. 5xFAD in different stages

(A) Workflow summarizing the process of mass-spectrum based proteomics.

(B) PCA of proteome data showed main difference in age (3-month-old: WT = 4, 5xFAD = 5; 4-month-old: WT = 4, 5xFAD = 4; 6-month-old: WT = 4, 5xFAD = 5; 9-month-old: WT = 3, 5xFAD = 7; 11-month-old: WT = 7, 5xFAD = 4).

(C) Diagram of DEPs in different month-old mice, degree of red color indicated their p values, nodes size indicates their log2FC, bar in nodes indicate their overlapping status among different month-old.

(D) GO pathway enrichment of DEPs of different month-old mice. The key pathways were colored in green, blue, red fonts which show specific enrichment in different month-old and purple box showed synapse related functions.

(E) GSEA enrichment of DEPs in different month-old mice. Results from left to right were sorted in the order of 3-month to 11-month.

(F) Classic synaptic proteins expression showed synapse impairment in 4-month-old 5xFAD. Left, Western blot images; right, quantification of western blots, t-test, ns, not significant; ∗p < 0.05, ∗∗∗p < 0.001. Data are presented as mean ± SEM.