TABLE 2.
Plasmids
| Plasmid | Markers | Source or reference |
|---|---|---|
| pRS316 | CEN URA3 | 33 |
| pLAK40 | pRS316 + SMK1 | 20 |
| YEp352 | 2μm URA3 | 18 |
| pMC1871 | lacZ | 6 |
| pLAK42 | YEp352 + SMK1 | 20 |
| pJS1 | YEp352 + HOP1/SMK1 | This study |
| pMDP83 | YEp352 + SMK1/lacZ (−219) | This study |
| pMDP86 | YEp352 + SMK1/lacZ (−181) | This study |
| pMDP89 | YEp352 + SMK1/lacZ (−139) | This study |
| pMDP126 | YEp352 + SMK1/lacZ (−124) | This study |
| pMDP95 | YEp352 + SMK1/lacZ (−96) | This study |
| pMDP92 | YEp352 + SMK1/lacZ (−71) | This study |
| pMDP119 | pMDP89 + pseudo-urs1S | This study |
| pMDP174 | pMDP89 + mseS | This study |
| pMDP176 | pMDP89 + pseudo-urs1S mseS | This study |
| pRS406 | URA3 | 33 |
| pMDP149 | pRS406 + SMK1 (−124) | This study |
| pMDP152 | pMDP149 + C118A | This study |
| pMDP199 | pRS406 + SMK1 (−139) | This study |
| pMDP183 | pMDP199 + pseudo-urs1S | This study |
| pMDP185 | pMDP199 + mseS | This study |
| pMDP187 | pMDP199 + pseudo-urs1S + mseS | This study |
| pMDP113 | pMDP89 + UASH | This study |
| pAV124 | 2μm URA3 HOP1/lacZ − URS1H | 39 |
| pCC51 | pAV124 + URS1H | 15 |
| pJX42 | pAV124 + pseudo-URSS | This study |
| pJX43 | pAV124 + MSES | This study |
| pAV130 | 2μm URA3 HOP1/lacZ − UASH | 39 |
| pCC83 | pAV130 + UASH | 16 |
| pJX33 | pAV130 + UASH | This study |
| pAV73 | 2μm URA3 CYC1/lacZ | 39 |
| pJX49 | pAV73 + MSES | This study |