TABLE 3.

Sporulation phenotypes of smk1 promoter mutants^a

^aDiploid smk1-Δ strains containing a single copy of the indicated smk1 promoter constructs were sporulated, and the fraction of cells that formed spores, as assayed by phase-contrast microscopy (n > 200), and fractional resistance to glusulase, ether, or heat shock treatment were determined as described in Materials and Methods. Completion of meiosis was greater than 84% in all samples, as monitored by fluorescence microscopy of DAPI-stained samples. The smk1 promoter mutants were generated by using the following integrating plasmids, from top to bottom: wild-type (pMDP149), pseudo-urs1^S (pMDP183), −118A substitution (pMDP152), mse^S (pMDP185), and pseudo-urs1^S mse^S (pMDP187). These strains are identical to those assayed by Northern blot analysis, for which results are shown in Fig. 3B and 5. Values are averages from at least two separate experiments.