TABLE 4.

Sporulation phenotypes of strains expressing SMK1 as an early gene^a

Plasmid	Fraction of cells forming spores	Fraction of cells with resistance to:
		Glusulase	Ether	Heat shock
2μm	0.00	<1.0 × 10−3	<1.0 × 10−3	<1.0 × 10−3
2μm SMK1	0.63	1.0	0.31	0.72
2μm HOP1/SMK1	0.53	1.2	0.85	0.45

^aThe smk1-Δ yeast strain MDPY10, harboring a 2μm control plasmid (YEp352), a 2μm plasmid containing the entire SMK1 gene (PLAK40), and a 2μm plasmid containing the HOP1 promoter directing the expression of SMK1 (pJS1) were sporulated, and the fractions of cells that completed sporulation or that were resistant to the indicated treatment were assayed as described for Table 3 and in Materials and Methods. Values are averages from at least two separate experiments.