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Quantification of ventricular zone
(A) Immunofluorescence of cerebral organoid cross-section, co-stained with SOX2 in red and Hoechst 33342 nuclear stain in blue. Yellow boxes outline example neural rosettes used to quantify the average size of the ventricular zone. Scale bar 100 μm.
(B) and (C) Depiction of measurements taken to evaluate the thickness of rosettes boxed in (A). Scale bar 20 μm in both.
