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Quantification of radial glia and intermediate progenitor cells
(A) Neural rosette of cerebral organoid cross-section, co-stained with PAX6 in red, TBR2 in green, and Hoechst 33342 nuclear stain in blue. Dashed yellow line traces border of rosette. Scale bar 50 μm. Individual channels are shown in (B) PAX6 in red, and (C) TBR2 in green. Yellow rectangle defines the region of interest for counting PAX6+ and TBR2+ positive nuclei using Cell Counter.
