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Quantification of early neuron fate progression
(A) Immunofluorescence of cerebral organoid cross-section, co-stained with TBR1 in red, CTIP2 in green, and Hoechst 33342 nuclear stain in blue. Scale bar 100 μm. Individual red and green channels shown in (B) and (C), respectively. Yellow box 200 μm by 200 μm is zoomed in in (D) merged, (E) red channel, and (F) green channel.
(G) Path and conditions to use the JACoP plug-in in FIJI/ImageJ, used to quantify colocalization.
