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Gelatin Solution
| Reagent | Final concentration | Amount |
|---|---|---|
| Sucrose | 10% | 10 g |
| D-PBS w/o Ca2+, Mg2+ | - | 100 mL |
| Gelatin | 7.5% | 7.5 g |
Add 10 g of sucrose to 100 mL of D-PBS and dissolve. Add 7.5 g of gelatin to the sucrose solution and mix thoroughly. Keep gelatin solution in a water bath at 55°C until ready to use to avoid solidification. This is made fresh for each use; do not store.
