Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Citrate Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tri-sodium citrate | 10 μM | 2.94 g |
| dH2O | - | 1 L |
| HCl | - | - |
| NaOH | - | - |
| Tween 20 | 0.5% | 0.5 mL |
Add 2.94 g of Tri-sodium citrate (dihydrate) to 950 mL of dH2O and dissolve completely. Adjust pH to 6.0 with HCl or NaOH. Add 0.5 mL of Tween 20 and top the entire volume up to 1 L using dH2O. Mix thoroughly. Store at a temperature between 15°C and 25°C for up to 3 months, or at 4°C for longer.
